在protected void CheckBox1_CheckedChanged事件中写代码： 

protected void CheckBox1_CheckedChanged(object sender, EventArgs e)

{

      CheckBox check = (CheckBox)sender; 

        //System.Web.UI.WebControls.TableCell dcf = (System.Web.UI.WebControls.TableCell)check.Parent;

        //DataGridItem dgItem = (DataGridItem)dcf.Parent;         DataGridItem dgItem = (DataGridItem)check.Parent.Parent;         string id = dgItem.Cells[1].Text;         if (check.Checked)         {

            string ID = dgItem.Cells[1].Text;

            string name = dgItem.Cells[2].Text;

        }       

}

附加：如果要遍历Datagrid中的CheckBox是否选中

     for (int j = 0; j < Datagrid.Rows.Count; j++)

    {

         CheckBox chbox = Datagrid.Rows[j].FindControl("CheckBox1") as CheckBox;

          if (chbox.Checked)

           {

                string ID = Datagrid.Rows[j].Cells[1].Text;

                string name = Datagrid.Rows[j].Cells[2].Text;

           }

     }